| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| TB 305-10 | ACE Reagent | 10x10 ml | 1-3 days | x |
ACE ReagentACE Reagent is for use in the kinetic determination of angiotensin converting enzyme (ACE) activity in serum or plasma at 340 nm. Angiotensin converting enzyme (ACE, EC3.4.15.1, dipeptidyl carboxypeptidase) is a glycoprotein peptidyldipeptide hydrolase that cleaves histidylleucine dipeptide from angiotensin I, a relatively inactive decapeptide. The latter is converted to the potent vasoconstrictor, angiotensin II. ACE also inactivates bradykinin. Elevated levels of ACE activity occur in serum of patients with active sarcoidosis, and occasionally in premature infants with respiratory distress syndrome, in adults with tuberculosis, Gaucher’s disease, leprosy, and in many other patogic conditions involving lung and liver diseases. Significantly low levels were reported by Siefkin et al., in many acute and chronic cases of lung injuries. Serial measurements of ACE in 71 patients showed that significantly decreasing levels over successive days were associated with a very high mortality rate. A single ACE measurement does not necessarily predict the presence or extent of lung injury, or aid in diagnosis of prognosis. However, serial levels are of value prognostically. Several methods have been devised for measuring ACE activity including radioimmunoassay and competitive enzyme-linked immunoassay. The procedure described herein is a rapid, convenient spectrophotometric method utilizing the synthetic tripeptide substrate N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG). |
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