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G-6-PDH

Order number Description Quantity Delivery time CE
TB 345-A G-6-PDH Reagent 20 tests 1-3 days x

G-6-PDH Reagent

Trinity Biotech Glucose-6-Phosphate Dehydrogenase reagents are for the quantitative, ultraviolet, kinetic determination of G-6-PDH in blood at 340 nm.

Glucose-6-phosphate dehydrogenase (G-6-PDH, D-glucose-6-phosphate: oxidoreductase, EC 1.1.1.49) catalyzes the first step in the pentose phosphate shunt, oxidizing glucose-6-phosphate (G-6-P) to 6-phosphogluconate (6-PG) and reducing NADP to NADPH. The Trinity Biotech procedure is a modification of the spectrophotometric methods of Kornberg and Horecker and of Lohr and Waller, involving the following reaction:

Nicotinamide adenine dinucleotide phosphate (NADP) is reduced by G-6-PDH in the presence of G-6-P. The rate of formation of NADPH is proportional to the G-6-PDH activity and is measured spectrophotometrically as an increase in absorbance at 340 nm. Production of a second molar equivalent of NADPH by erythrocyte 6-phosphogluconate dehydrogenase (6-PGDH) according to the reaction:

is prevented by use of maleimide, an inhibitor of 6-PGDH.



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