| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| TB 735-10 | Lactate Reagent | 10x10 ml | 15-20 days | x |
Lactate ReagentLactate Reagent is for the quantitative, enzymatic determination of lactate in plasma at 540 nm. Lactic acid is a by-product of carbohydrate metabolism. Blood lactate arises primarily from muscle cells and erythrocytes and is metabolized by the liver. Therefore, blood lactate levels reflect both production and metabolism. Severe tissue oxygen deprivation leads to “lactic acidosis” characterized by weakness, stupor, fatigue and coma. The liver can normally remove more lactate than the body can produce, hence treatment for hypoxia and removal of the initiating condition usually results in lowering of the blood lactate. Lactate measurement also can be useful clinically in the diagnosis of angina pectoris or in liver function testing where reduced liver function is suspected. Both lactate and pyruvate levels provide an index of severity of circulatory failure. Several types of lactic acidosis based on association with tissue hypoxia, systemic disorders, drug and toxin ingestion, neoplastic proliferative disorders and inborn errors of metabolism have been described. Lactate metabolism and post-exercise oxygen consumption have been extensively studied from as early as 1900. The contemporary view holds that “Lactate metabolism regardless of pathways is occurring at all times after exercise and thus cannot be associated with a particular phase of the post-exercise VO2”. Early analytical methods for lactate lacked both specificity and sensitivity. The availability of highly purified nicotinamide adenine dinucleotide (NAD) and its reduced form (NADH), and lactate dehydrogenase (LD) led to the development of highly specific assays for both lactate and pyruvate. An enzymatic procedure for lactate based on the reduction of NAD to NADH by lactate dehydrogenase is detailed in Trinity Biotech Procedure No. 826-UV. That procedure is carried out at 340 nm. |
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