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Lipase

Order number Description Quantity Delivery time CE
TB 805-A Lipase-PS Kit 3x10 ml 15-20 days x

Lipase-PS Kit

Lipase-PS™ Reagent is intended for the quantitative, kinetic determination of pancreatic lipase (triacylglycerol acylhydrolase, E.C. 3.1.1.3) activity in serum at 550 nm.

The measurement of serum lipase activity is widely used for the diagnosis of acute pancreatitis. A 10-fold increase of lipase activity above the upper reference limit is suggestive of pancreatitis, pancreatic injury, or inflammation of organs contiguous to the pancreas. It is recommended that other tests, such as trypsinogen and amylase isoenzymes be performed to supplement the diagnosis. Pancreatic lipase hydrolyzes the α-1 and α-3 esters of long chain fatty acids from their triglycerides. The enzymic activity requires the presence of co-lipase which facilitates the binding of substrate to the enzyme. The Cherry-Crandall titrimetric method is based on the titration of fatty acids, whereas in the turbidi-metric method of Shihabi and Bishop pancreatic lipase activity is monitored by the decrease of turbidity in the triglyceride emulsion. Other approaches to lipase assay include colorimetric, nephelometric, and fluorometric methods.
The Trinity Biotech procedure is based on the colorimetric method of Imamura, et al. 1,2-Diglyceride is hydrolyzed to 2-monoglyceride and fatty acid. 2-Monoglyceride is then measured by coupled enzyme reactions catalyzed by monoglyceride lipase, glycerol kinase, glycerolphosphate oxidase, and peroxidase. The test is highly sensitive and specific for pancreatic lipase using co-lipase and deoxycholate as activators. The assay is simple to perform and can easily be adapted to automated analyses.



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