| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| TB 591-D | Oxalate Kit | 100 tests | 1-3 days | x |
Oxalate KitTrinity Biotech Oxalate reagents are for the quantitative, enzymatic determination of oxalate in urine at 590 nm. Oxalate was confirmed as a normal constituent of urine in 1951, but only recently has the significance of calcium oxalate crystalluria and its relationship to urinary tract stone formation been fully recognized. Formation of the sparingly soluble calcium salt of oxalate in the urinary tract is considered the major factor in urolithiasis. Oxalate in urine may arise either as an endproduct of intermediary metabolism or from dietary sources. A decreased excretion of oxalate in the urine is associated with hyperglycinemia and hyperglycinuria. An increased excretion of oxalate can be attributed to increases in ingestion of oxalate precursors or oxalate rich foods, formation of oxalate due to metabolic defects such as in primary hyperoxaluria, and absorption of oxalate in a number of gastrointestinal disorders that produce severe fat malabsorption. This latter group includes patients with inflammatory bowel disease, ileal resection, biliary diversion, pancreatic insufficiency, sprue, small intestinal stasis with bacterial overgrowth, and following jejunoileal bypass or resection for the treatment of obesity. Currently, urinary oxalate determination is performed by procedures based on isotope dilution, gas and ion chromatography, as well as coupled enzyme reactions. These procedures are very time consuming and may require equipment not readily available in the clinical laboratory. The enzymatic method described below is based on the oxidation of oxalate by oxalate oxidase followed by measurement of hydrogen peroxide (H 2O2) produced during the reaction by a peroxidase-catalyzed reaction. The procedure is specific for oxalate. It requires no special equipment and is easily adaptable for use on clinical automated analyzers. |
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