| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| TB T6004 | TriniLIZE PAI-1 Activity | 96 tests | 20-25 days | x |
TriniLIZE PAI-1 ActivityTriniLIZE PAI-1 Activity is a bio immunoassay (BIA) for the quantitative determination of active human plasminogen activator inhibitor, type 1 (PAI-1) in human plasma. An increased plasma level of PAI-1 is an important reason for impaired fibrinolytic function and may be associated with thrombotic diseases. Increased levels of PAI-1 have also been found in conditions such as normal pregnancy6 and sepsis. TriniLIZE PAI-1 Activity assay is an immunoactivity assay. Active tPA is lyophilized while immobilised on the surface of the microtest well. After reconstitution, plasma samples containing PAI-1 are added and active PAI-1 reacts with the bound tPA. Simultaneously, an HRPconjugated monoclonal antibody (MA 12A4, see reference 8) to PAI-1 is added. After incubation, unbound sample and conjugated antibody are washed away and the bound PAI-1 quantified with an HRP-sensitive substrate. The amount of colour developed is directly proportional to the concentration of active PAI-1 in the sample. One unit of PAI-1 activity is defined as the amount of PAI-1 that inhibits one international unit of human single chain tPA as calibrated against the International Standard for tPA, lot 86/670 distributed by NIBSC, Holly Hill, London, England. The TriniLIZE PAI-1 Activity assay is standardised against the NIBSC PAI-1. |
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