| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| TB T6003 | TriniLIZE PAI-1 Antigen | kit | 15-20 days | x |
TriniLIZE PAI-1 AntigenTriniLIZE PAI-1 Antigen is an enzyme immunoassay (ELISA) for the quantitative determination of human plasminogen activator inhibitor, type 1 (PAI-1) antigen in human plasma. Plasminogen activator inhibitor; type 1 (PAI-1) is the primary inhibitor of tissue plasminogen activator (tPA) a key enzyme in fibrinolysis. An increased plasma level of PAI-1 is associated with impaired fibrinolytic function. Elevated levels of PAI-1 have been observed in thrombolytic disease, acute myocardial infarction, deep vein thrombosis as well as normal pregnancy and sepsis. PAI-1 is produced by vascular endothelial cells, platelets, placenta, and kidney tubule cells. PAI-1 is found in several forms: active inactive (latent) and complexed to tPA and uPA all of which are measured in the TriniLIZE PAI-1 assay. The TriniLIZE PAI-1 Antigen test is based on a double antibody principle similar to the ELISA described by Declerck et al. and uses the same coating antibody (MA-7D4B7). The assay uses quenching and normal antibodies to exclude falsely elevated results from the specific response a limitation of conventional ELISA assay. This method to obtain specific ELISA is “ISAC” (immunological Specificity and Accuracy Control; patented). The A-wells (green in colour) contain monoclonal antibodies against PAI-1 immobilized onto the well surface as well as soluble antibodies against PAI-1. The N-wells (blue in colour) contain the same monoclonal antibodies against PAI-1 onto the well surface and soluble non-immune antibodies. During sample incubation, the PAI-1 antigen in the sample is quantitatively bound to antibodies coating the N-wells, but not the A-wells (prevented from binding by the soluble anti-PAI-1 antibodies). HRP-conjugated anti-PAI-1 antibodies are added and will also bind to the PAI-1 molecule. This forms a sandwich of coating antibody: PAI-1: conjugate antibody. The wells are washed to remove excess conjugate and other unbound material. The HRP substrate (OPD/H2O2) is added and converted to the yellow-collared product at a rate proportional to the amount of HRP-conjugate bound. The difference in response between the N-well and the A-well is the specific PAI-1 response. |
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