| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| TB T6001 | TriniLIZE tPA Antigen | kit | 15-20 days | x |
TriniLIZE tPA AntigenTriniLIZE tPA Antigen is intended for quantitative determination of human tissue plasminogen activator antige (tPA) in plasma by enzyme immunoassay. The clinical utility of the assay is to detect disorders of the fibrinolytic system. Tissue-type plasminogen activator (tPA) constitutes an important agent in the fibrinolytic pathway and the levels of tPA are thought to have a major effect on fibrinolytic potential. The physiological role of tPA is to activate plasminogen to plasmin, which in turn degrades fibrin to soluble degradation products. Two forms of the tPA molecule are present in plasma, the singlechain and the two-chain form, and both forms are equally capable of activating plasminogen. Fibrinolysis is regulated by specific molecular interactions between tPA and fibrin as well as between plasmin and the specific plasmin inhibitor, α-2-antiplasmin. The TriniLIZE tPA Antigen test utilises the double antibody principle. Plasma sample or standard containing tPA is added to a microtest well which is coated with goat anti-tPA IgG and contains soluble non-immune goat IgG. After an incubation sufficient to allow >95% of the tPA to bind to the capture antibodies, HRP-labelled Fab fragments of anti-tPA IgG are added. These are allowed to react with the bound tPA. The wells are emptied and washed to remove unbound conjugate after which peroxidase substrate (OPD/H2O2) is added. The amount of yellow colour developed is directly proportional to the amount of tPA present in the sample. The tPA antigen standard, provided with the TriniLIZE tPA Antigen kit, contains human single-chain tPA and is calibrated against the international standard for tPA, lot 86/670 distributed by NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, England |
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