| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| APCPCI-EIA | APC-Protein C Inhibitor Complex EIA Set | 5 plates | 7-10 days |
APC-Protein C Inhibitor Complex EIA SetActivation of coagulation leads to the generation of thrombin which, in the presence of thrombomodulin, will activate Protein C to the enzyme activated Protein C (APC). Unless regulated, APC will exert its anticoagulant function through proteolytic inactivation of factor Va and factor VIIIa. In blood, the activity of APC is regulated in part through interaction with protease inhibitors to form inactive enzymeinhibitor complexes. Based on physiological concentrations and the kinetics of inhibition, the primary inhibitor of APC in blood is Protein C Inhibitor (PCI, also known as plasminogen activator inhibitor-3), followed by á1antitrypsin (á1AT, also known as á1proteinase inhibitor) and á2macroglobulin. The APC-PCI complex (APC-PCI) results when APC cleaves a scissile bond near the C-terminus of PCI, forming a covalent, 1:1 acyl enzyme intermediate with PCI with an apparent mass of 110 kDa. Calcium is not required for this interaction, but the rate of APC inhibition by PCI can be accelerated 50-fold by optimal concentrations of heparin. APC-PCI complex is cleared from circulation with a half-life of 19 minutes, presumably by serpinenzyme complex receptors on the surface of hepatocytes. |
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