| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| APCAT-EIA | [APC-a1-Antitrypsin] complex EIA Kit | 5 plates | 7-10 days |
[APC-a1-Antitrypsin] complex EIA KitActivation of coagulation leads to the generation of thrombin which, in the presence of thrombomodulin, will activate Protein C to the enzyme activated Protein C (APC). Unless regulated, APC will exert its anticoagulant function through proteolytic inactivation of factor Va and factor VIIIa. In blood, the activity of APC is regulated in part through interaction with protease inhibitors to form inactive enzymeinhibitor complexes. Based on physiological concentrations and the kinetics of inhibition, the primary inhibitor of APC in blood is Protein C Inhibitor (PCI, also known as plasminogen activator inhibitor-3), followed by á1antitrypsin (á1AT, also known as á1proteinase inhibitor) and á2macroglobulin. The APC-á1AT complex (APCAT) results when APC cleaves a scissile bond near the C-terminus of á1AT, forming a covalent, 1:1 acyl enzyme intermediate with á1AT with an apparent mass of 110 kDa. Calcium is not required for this interaction and there is no significant rate enhancement in the presence of heparin. The clearance rate for APCAT clearance from the circulation (half-life of 72 minutes) is slow relative to clearance rates reported for APC-PCI as well as thrombin-antithrombin and thrombin-heparin cofactor II complexes (half-lives of 10-19 minutes). The prolonged survival of APCAT may be an asset in the use of APCAT as a marker of Protein C activation. |
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