| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| THCII-EIA | Thrombin-Hep. Co-II Complex EIA set | 5 plates | 7-10 days |
Thrombin-Hep. Co-II Complex EIA setThe activation of coagulation ultimately leads to the activation of prothrombin to the enzyme thrombin. Unless regulated, thrombin will act on its natural substrates that include fibrinogen, factor V, factor VIII, factor XIII, Protein C, TAFI as well as specific receptors on platelets and endothelial cells. The activity of thrombin in plasma is regulated in part through interaction with protease inhibitors. Based on kinetic rates and physiological concentrations, the primary inhibitor of thrombin in plasma is antithrombin (ATIII), followed by heparin cofactor II (HCII) and á2macroglobulin. The thrombin-heparin cofactor II complex (T-HCII) results when thrombin cleaves a scissile bond near the C-terminus of HCII, forming a covalent, 1:1 acyl enzyme intermediate with HCII with an apparent mass of 102 kDa. Calcium is not required for this interaction, but the rate of thrombin inhibition by HCII can be accelerated 1000-fold by optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. T-HCII complexes are cleared from circulation by serpin-enzyme complex receptors on the surface of hepatocytes, with a half-life of 10 minutes. |
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