| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| CO 026-001 | Anti-Cardiolipin IgA ELISA | 1 plate | 10-15 days |
Anti-Cardiolipin IgA ELISAFor the detection and semi-quantitation of anti-cardiolipin antibodies in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (anti-phospholipid syndrome). The test is performed as an indirect ELISA. Diluted serum samples, calibrator sera, and controls are incubated in cardiolipin coated microwells, allowing aCL antibodies present in the samples to react with the immobilized antigen. After the removal of unbound serum proteins by washing, antibodies specific for human IgA labeled with horseradish peroxidase (HRP) are added forming complexes with the cardiolipin bound antibodies. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of tetramethylbenzidine Results are obtained by reading the O.D. (optical density or absorbance) of each well with a spectrophotometer. Calibrator sera are provided, with the IgA aCL concentration expressed in APL units. The user has the option of running either a single point calibrator or a four-point calibration curve. For single point calibration, dividing the concentration value of the calibrator by the O.D. value of the calibrator provides a conversion factor. The O.D. values of the controls and patient samples are multiplied by the conversion factor to obtain IgA aCL values, |
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