| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| CO 030-001 | Anti-Phosphatidylserin IgG/IgM ELISA | 1 plate | 10-15 days |
Anti-Phosphatidylserin IgG/IgM ELISADetection and semi-quantitation of anti-phosphatidylserine antibodies in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (anti-phospholipid syndrome). The test is performed as an indirect ELISA. The concentration of IgG anti-phosphatidylserine antibodies and IgM anti-phosphatidylserine antibodies must be determined separately. Diluted serum or plasma samples, calibrator sera, and controls are incubated in phosphatidylserine coated microwells. B2- glycoprotein I is provided in the sample diluent. Incubation allows the anti-phosphatidylserine (aPS) antibodies present in the samples to react with the immobilized antigen. After the removal of unbound serum or plasma proteins by washing, antibodies specific for human IgG or IgM, labeled with horseradish peroxidase (HRP), are added forming complexes with the phosphatidylserine bound antibodies. Two enzyme-conjugated antibody solutions are provided, one specific for human IgG antibodies and one specific for human IgM antibodies. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of a single solution containing tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) as the chromogenic substrate. Color develops in the wells at an intensity |
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