| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| CO 10206 | Anti-Phosphatidylserin IgA ELISA | 1 plate | 10-15 days |
Anti-Phosphatidylserin IgA ELISADetection and semi-quantitation of IgA anti-phosphatidylserine (aPS) antibodies as an aid for assessing the risk of thrombosis in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (anti-phospholipid syndrome). The test is performed as an indirect ELISA. Diluted serum or citrated plasma samples, calibrator sera, and controls are incubated in phosphatidylserine coated microwells. B2-glycoprotein I is provided in the sample diluent. Incubation allows the anti-phosphatidylserine (aPS) antibodies present in the samples to react with the immobilized antigen. After the removal of unbound serum or plasma proteins by washing, antibodies specific for human IgA, labeled with horseradish peroxidase (HRP), are added forming complexes with the phosphatidylserine bound antibodies. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of a single solution containing tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) as the chromogenic substrate. Color develops in the wells at an intensity proportional to the serum concentration of IgA antiphosphatidylserine (aPS) antibodies. |
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