| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| CO 038-001 | Anti-B2 GPI IgM ELISA | 1 plate | 10-15 days |
Anti-B2 GPI IgM ELISAFor the detection and semi-quantitation of IgM anti-B2GPl antibodies in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (anti-phospholipid syndrome). The test is performed as an indirect ELISA. Diluted serum or plasma samples, calibrator sera, and controls are incubated in microwells coated with purified human B2GPl. Incubation allows the anti-B2GPl antibodies present in the samples to react with the immobilized antigen. After the removal of unbound serum or plasma proteins by washing, antibodies specific for human IgM, labeled with horseradish peroxidase (HRP), are added forming complexes with the B2GPl bound antibodies. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of a single solution containing tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) as the chromogenic substrate. Color develops in the wells at an intensity proportional to the serum concentration of anti-B2GPl antibodies. |
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