| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| CO 039-001 | Anti-B2 GPI IgA ELISA | 1 plate | 10-15 days |
Anti-B2 GPI IgA ELISAFor the detection and semi-quantitation of IgA anti-B2GPl antibodies in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (anti-phospholipid syndrome). The test is performed as an indirect ELISA. Diluted serum or plasma samples, calibrator sera, and controls are incubated in microwells coated with purified human B2GPl. Incubation allows the anti-B2GPl antibodies present in the samples to react with the immobilized antigen. After the removal of unbound serum or plasma proteins by washing, antibodies specific for human IgA, labeled with horseradish peroxidase (HRP), are added forming complexes with the B2GPl bound antibodies. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of a single solution containing tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) as the chromogenic substrate. Color develops in the wells at an intensity proportional to the serum concentration of anti-B2GPl antibodies. Results are obtained by reading the O.D. (optical density or absorbance) of each well in a spectrophotometer. Calibrator sera are provided, with the IgA anti-B2GPl antibody concentrations expressed in A units. The user has the option of running either a single point calibrator or a four-point calibration curve. For single point calibration, dividing the concentration value of the calibrator sera by the O.D. value of the calibrator provides a conversion factor. The O.D. values of the other samples are multiplied by the conversion factor to obtain IgA anti-B2GPl antibody concentrations in A units. For multipoint calibration, perform a linear regression analysis with calibrator values against calibrator O.D.s. Controls and patient results are determined from the calibration curve. |
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