| Order number | Description | Quantity | Delivery time | CE |
|---|---|---|---|---|
| TB T1602 | TriniCLOT Protein S | kit | 15-20 days | x |
TriniCLOT Protein STriniCLOT Protein S is intended for the quantitative determination of Protein S activity in human plasma. The TriniCLOT Protein S assay is a clotting-based plasma assay. In the assay, dilutions of patient plasma are mixed with Protein S depleted plasma. A reagent that contains Factor Xa, activated Protein C and phospholipids is added to the mixture. Following a 5 minute incubation period, calcium chloride is added to initiate clot formation. Under these conditions, the prolongation of the clotting time is directly proportional to the concentration of the Protein S in the patient plasma. DiScipio and colleagues1 first identified human Protein S (S for Seattle) in 1977. Human Protein S is a single chain glycoprotein of 635 amino acids (69,000 kD) that circulates in plasma at a concentration of about 320 nM. The active Protein S gene has 15 exons and spans more than 80 kilobases on Chromosome 3. Protein S is a Vitamin Kdependant protein primarily synthesized in the liver but also in the endothelium and possibly in megakaryocytes. It is possible that all three sources of Protein S play a significant role in regulating hemostasis. Protein S did not appear to be the zymogen precursor of a serine proteinase and no function was known until 1980 when Walker reported that the bovine Protein S functions as a cofactor for activated Protein C in the inactivation of activated Factor V. The biological importance of the regulation of coagulation by the pathway involving Protein C, Protein S and thrombomodulin was soon substantiated by clinical studies. An association between thrombosis and partial deficiencies of Protein S was documented in certain families Protein S was also found on the surface of endothelial cells and platelets. Approximately 60% of Protein S in human plasma is bound tightly to C4bbinding protein. Therefore, plasma levels of Protein S may not completely reflect its bioavailability. This complex has no anticoagulant cofactor activity. It has been |
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