Login Login     en | fr
Welcome to Kordia Life Sciences, your partner in coagulation and hemostasis. Our customers will come first!
Productgroups
Product Search:

Specialty kits

Order number Description Quantity Delivery time CE
CO 12136 AspirinWorks 1 kit 10-15 days

AspirinWorks

The AspirinWorks® Test Kit is a quantitative enzyme-linked immunoassay (ELISA) to determine levels of 11-Dehydro Thromboxane B2 (11dhTxB2) in human urine, which aids in the determination of platelet response to aspirin ingestion.

The AspirinWorks® Test Kit measures urinary 11dhTxB2 and is performed as a competitive ELISA. Diluted samples (Reference Solution, controls, and patient urine), purified 11dhTxB2 conjugated to alkaline phosphatase (AP), and purified mouse monoclonal antibody directed to 11dhTxB2 are combined and incubated in microwells coated with a polyclonal anti-mouse antibody. Incubation allows the endogenous 11dhTxB2 present in the samples to compete with the purified AP-conjugated 11dhTxB2 for binding to the mouse monoclonal anti-11dhTxB2 antibody. The monoclonal antibody then binds to the polyclonal antimouse antibody coated on the microtiter plate. The complex formed on the plate is composed of monoclonal antibody and endogenous or AP-conjugated 11dhTxB2. After the removal of unbound complexes by washing, the bound AP-11dhTxB2 conjugate is assayed by the addition of paranitrophenylphosphate
(pNPP) chromogenic substrate. Color develops in the wells at an intensity inversely proportional to the sample urine concentration of 11dhTxB2, and is read at 405nm. Results (pg/mL) are calculated against a reference curve prepared from the Reference Solution provided in the kit. Final Results are reported as pg 11dhTxB2 per mg creatinine to normalize results for urine concentration.



   Kordia    PO Box 4018    2301 RA  LEIDEN    The Netherlands    Tel.: +31 (0)71 523 10 50    Fax: +31 (0)71 522 17 00