Login Login     en | fr
Welcome to Kordia Life Sciences, your partner in coagulation and hemostasis. Our customers will come first!
Productgroups
Product Search:

Specialty kits

Order number Description Quantity Delivery time CE
CO 029-001 Hyaluronic Acid Microplate Test Kit 1 plate 10-15 days

Hyaluronic Acid Microplate Test Kit

An enzyme-linked binding protein assay for the determination of hyaluronic acid (HA) in human serum or plasma.

The HA test kit is an enzyme-linked binding protein assay that uses a capture molecule known as hyaluronic acid binding protein (HABP).22,23 Properly diluted serum or plasma and HA reference solutions are incubated in HABP-coated microwells, allowing HA present in samples to react with the immobilized binding protein (HABP). After the removal of unbound serum molecules by washing, HABP conjugated with horseradish peroxidase (HRP) solution is added to the microwells to form complexes with bound HA. Following another washing step, a chromogenic substrate of tetramethylbenzidine and hydrogen peroxide is added to develop a colored reaction. The intensity of the color is measured in optical density (O.D.)
units with a spectrophotometer at 450nm. HA levels in patient and control samples are determined against a reference curve prepared from the reagent blank (0 ng/mL) and the HA reference solutions provided with the kit (50, 100, 200, 500, 800 ng/mL).



   Kordia    PO Box 4018    2301 RA  LEIDEN    The Netherlands    Tel.: +31 (0)71 523 10 50    Fax: +31 (0)71 522 17 00