TECHNOZYM® t-PA Ag EDTA ELISA
The TECHNOZYM® t-PA Antigen ELISA can be used to determine t-PA antigen levels in patients with thrombotic disorders (deep vein thrombosis, myocardial infarction, stroke), malignancies or septicaemia.
COMPOSITION
1. ELISA test strips (12) with 8 wells each, coated anti t-PA monoclonal antibody; the drying agent is supplied in an aluminium bag.
2. Washing buffer concentrate (PBS; pH 7.3); containing detergent; 0.01% merthiolate; 1 bottle, 100 ml.
3. Incubation buffer (PBS; pH 7.3); contains stabiliser protein; 0.05% proclin; and blue dye, 1 bottle, 100 ml, ready for use.
4. Sample dilution buffer (PBS, EDTA); contains stabiliser protein; 1 bottle, 20mL
5. Calibrators (Standards) numbered; lyophilised; 1 bottle each. Concentrations are lot-dependent; consult label on the vial.
6. Control plasmas "low level" and "high level" for checking purposes lyophilised; 1 bottle each. Concentrations are lot-dependent; consult the label on the vial.
7. Conjugate monoclonal Anti-t-PA POX; dyed blue; 1 bottle, 0.3 ml.
8. Chromogen TMB (tetramethylbenzidine); 1 bottle, 12 ml; ready for use.
9. Stopping solution sulphuric acid 0.45 mol/l; 1 bottle 12ml; ready for use.
10. Adhesive film: for ELISA test strips (2).
REFERENCE RANGE
Normal range for t-PA antigen is between 2- 8 ng/ml It is recommended that individual laboratories establish their own ranges. For plasma obtained after venous occlusion values greater than +10ng/ml, should be obtained. A failure of t-PA levels to increase upon venous occlusion is indicative of an impaired fibrinolytic capacity and may indicate thrombotic tendencies.
|
Additional Documentation
|
Connection